Biochemistry MCQ Quiz Hub

1. An example of a transamination process is
glutamate = hexanoic acid + NH3
aspartate + hexanoic acid = glutamate + oxaloacetate
aspartate + α ketoglutarate = glutamate + oxaloacetate
glutamate = α-ketoglutarate + NH3

2. Transamination is the process where
carboxyl group is transferred from amino acid
α-amino group is removed from the amino acid
polymerisation of amino acid takes place
none of the above

3. The most toxic compounds is
tyrosine
phenylpyruvate
lysine
phenylalanine

4. A person with phenylketonuria is advised not to consume which of the following products?
Glycine containing foods
Fat containing food
Glucose
Aspartame

5. Tyrosine is degraded to acetoacetyl CoA and fumarate and is described as a
glucogenic amino acid
ketogenic amino acid
ketogenic and glucogenic amino acid
keto-gluco amino acid

6. Transaminase enzymes are present in
liver
pancreas
intestine
none of these

7. A person with phenylketonuria will convert
phenylalanine to phenylpyruvate
phenylalanine to isoleucine
phenylpyruvate to phenylalanine
tyrosine to phenylalanine

8. An example of the oxidative deamination is
glutamate = hexanoic acid + NH3
aspartate + α-ketoglutarate = glutamate + oxaloacetate
glutamate = α-ketoglutarate + NH3
aspartate + hexanoic acid = glutamate + Oxaloacetate

9. In the normal breakdown of phenylalanine, it is initially degraded to
fumarate
tyrosine
lysine
phenylpuruvate

10. Transamination is the transfer of an amino
acid to a carboxylic acid plus ammonia
group from an amino acid to a keto acid
acid to a keto acid plus ammonia
group from an amino acid to a carboxylic acid

11. Lysine is degraded to acetoacetyl CoA and is described as a
ketogenic amino acid
glucogenic amino acid
keto-gluco amino acid
none of these

12. A ketogenic amino acid is one which degrades to
keto-sugars
either acetyl CoA or acetoacetyl CoA
pyruvate or citric acid cycle intermediates
multiple intermediates including pyruvate or citric acid cycle intermediates and acetyl CoA or acetoacetyl CoA

13. A best described ketogenic amino acid is
lysine
tryptophan
valine
none of these

14. A person suffering from phenylketonuria on consumption food containing high phenylalanine may lead to the accumulation of
phenylalanine
phenylpyruvate
tyrosine
isoleucine

15. Which of the following mineral is involved in protein metabolism and oxidation reactions?
Molybdenum
Manganese
Magnesium
calcium

16. Which of the following is not a function of iron?
Oxygen transport
Immune function
Brain function
Gene regulation

17. Consumption of which nutrient leads to the multiplication of oral bacteria?
Fat
Carbohydrate
Protein
Fluoride

18. Examples of goitrogenic foods are __________ and __________ .
turnips and corn
rutabagas and okra
raw cabbage and turnips
cassava and lettuce

19. Which of the following carbohydrate is effective in promoting the calcium absorption?
Lactose
Maltose
Sucrose
Xylose

20. What condition is caused by iodine deficiency during pregnancy and is characterized by stunted growth, deafness, and mental retardation?
Cretinism
Keshan disease
Multiple sclerosis
Crohn's disease

21. Iodine is a part of thyroid hormone and is essential for the prevention of
goiter
osteoporosis
muscle weakness
diarrhea

22. What transports copper from the intestinal cells to the liver?
Ceruloplasmin
Secretin
Acrolein
Albumin

23. Which group is most often affected with copper deficiency?
Elderly woman
Alcoholics
Active toddlers with limited
Preterm infants food variety

24. What is the main source of natural fluoride?
Mushrooms
Potatoes
Meat
Water

25. Which of the following mineral is involved in the enzyme -controlled energy yielding reactions of metabolism?
Calcium
Phosphorus
Iron
Copper

26. The mineral which aids in the utilization of iron and in hemoglobin synthesis is
Calcium
Phosphorus
cobalt
Copper

27. The mineral which is considered important in maintaining electrical potential in nerves and membranes is
magnesium
manganese
Calcium
Iron

28. Wilson's disease is an example of __________ and Menkes' syndrome is an example of __________ .
zinc deficiency; zinc toxicity
zinc toxicity; zinc deficiency
copper deficiency; copper toxicity
copper toxicity; copper deficiency

29. Which of the following is correct?
Cobalt is part of vitamin B12.
Cobalt will not replace the need for vitamin B12 in humans
Both (a) and (b)
Cobalt will replace the need for vitamin B12 in humans

30. Which of the following is required as a component of blood hemoglobin?
Iron
Magnesium
Calcium
Copper

31. Adding __________ to a breakfast of cereal will help your body absorb iron.
milk
orange juice
coffe
water

32. Which of the following ion is required for the development of sound teeth with resistance to tooth decay?
Fluoride ion
Sodium ion
Chloride ion
Magnesium ion

33. Which of the following two beverages contribute 20 to 30 percent of our daily manganese intake?
Milk or tea
Cola or coffee
Tea or orange juice
Milk or orange juice

34. An endemic disease of cardiomyopathy in children is attributed to
low Se in the environment
low intake of iron
high Se in the environment
high intake of iron

35. Which of the following statement is correct?
Sodium and chloride are the chief extracellular ions of the body
Sodium and chloride are primarily involved in maintaining osmotic equilibrium and body fluid volume.
Chloride ion is necessary for the production of hydrochloric acid of gastric juice.
All of these

36. Which of the following is necessary for the clotting of blood, functions of certain enzymes and control of fluids through cell membranes?
Calcium
Magnesium
Copper
All of these

37. Selenium deficiency leads to
liver necrosis
diarrhea
multiple sclerosis
Crohn's disease

38. Manganese is a key component of all of the following enzymes except
arginase
pyruvate carboxylase
ceruloplasmin
Mn-superoxide dismutase

39. Which of the following vitamin is essential for the absorption of calcium from the intestinal tract?
Vitamin D
Vitamin E
Vitamin B12
Vitamin B6

40. Which trace mineral is a component of the enzyme that activates vitamin A in the eye?
Zinc
Iron
Iodine
Chromium

41. Chronic consumption of excess selenium can lead to
brittle hair and nails.
muscle weakness,
TPN
diarrhea.

42. Normal glucose metabolism requires
chromium
manganese
magnesium
calcium

43. Manganese is needed for
normal bone structure
reproduction and functioning of central nervous system
both (a) and (b)
muscle strengthening

44. What is shielding in NMR?
Using a curved piece of metal to block an opponents attack
Putting metal around an Rf source
When the magnetic moment of an atom blocks the full induced magnetic field from surrounding nuclei
Blocking parts of a molecule from Rf radiation

45. What is used to cool the superconducting coil?
Hydrogen
Ice
Dry ice
Liquid helium

46. Coupling causes the peaks in 1H NMR spectra to be split into
two peaks
multiple peaks equal to the number of hydrogens on surrounding atoms
multiple peaks equal to the number of surrounding carbon atoms
multiple peaks equal to the number of hydrogen on surrounding atoms, plus one

47. When placed in a magnetic field, all the random spins of the nuclei
stop
reverse direction
align with the magnetic field
rotate to 90° away from the induced field

48. Better understanding of the nuclei is possible,
with the help of wavelength spectrum
with the help of frequencies ranges
with the help of a mathematical translator called the fourier transfer algorithm
none of the above

49. All hydrogen atoms
have the same resonance frequency
resonate at different frequencies depending on their environment.
are attached to carbon
. resonate at about the same frequency as carbon

50. Why is it important to use a deuterated solvent?
NMR uses least of this solvent
So the spectrometer can lock onto the sample to prevent the spectrum from drifting during aquisition
Expensive solvents work best with NMR
They dissolve polymer the fastest

51. Which of the following is the basis of first dimension of separation for two-dimensional electrophoresis?
Molecular mass
Solubility
Isoelectric point
Folding

52. What is meant by rotating frame of reference?
That the sample is spun rapidly in the applied field
If the laboratory itself is imagined to be rotated at the Larmor frequency, viewing that individual magnetic moment vectors are fixed in space
That the detector rotates around the sample
None of the above

53. The sequence of amino acids in proteins can be determined by means of
identification of the -NH2 terminal amino acids
identification of the -COOH terminal amino acids
partial cleavage of the original polypeptide into smaller polypeptides
all of the above

54. Why is it advantageous to record many FID signals from the same sample and then add them together?
To ensure that all target nuclei in the sample have been excited
To remove inaccuracies caused by fluctuations in the applied magnetic field
To increase sensitivity
None of the above

55. In FT-NMR, how are nuclei excited?
By radio-frequency radiation whose frequency is swept across a predetermined range
By an intense pulse of radiation which contains a wide range of frequencies
By an intense pressure
None of the above

56. What is the name given to the relaxation process due to an interaction between an excited nucleus and the magnetic fields caused by nuclei in molecules moving around in the sample?
Spin - lattice relaxation
Spin - spin relaxation
Spin - spin - spin relaxation
None of these

57. Cytochrome C has an isoelectric pH of
8.5
10.05
7.5
11.05

58. What does the Michelson interferometer do?
Split a polychromatic beam of radiation into its component wavelengths
Selectively filter certain wavelengths from a beam of I.R. radiation
Modulate the I.R. signal at a lower frequency, so that it can be observed by a detector
none of the above

59. In scanning electron microscopy
a specimen is fixed and then coated with thin layer of a heavy metal
a specimen is fixed and then coated with transition metal
a specimen is not fixed and then coated with thin layer of a heavy metal
none of the above

60. The frequency of precession, the transition frequency and the Larmor frequency are
different terms for the same frequency
same terms for the same frequency
different terms for the different frequency
same terms for the different frequency

61. How do you turn a signal recorded in the time domain into a frequency domain signal?
Fourier transformation
Measurement of peak areas
By use of a Michelson interferometer
None of the above

62. How many possible orientations do spin 1/2 nuclei have when they are located in an applied magnetic field?
2
4
3
6

63. In immunofluroscence microscopy, fluroscent compounds are attached to
an antibody specific for the subcellular structure
an antigen specific for the subcellular structure
bound antibody specific for the subcellular structure
none of these

64. An FT-IR instrument record a signal in the
time domain
frequency domain
both (a) and (b)
none of these

65. When radiation energy is absorbed by a spin 1/2 nucleus in a magnetic field, what happens?
The processional frequency of the nucleus increases
The nucleus spins faster
The angle of precession flips so that the magnetic moment of the nucleus opposes the applied field
none of the above

66. Negative staining is a technique used in
electron microscopy
gel electrophoresis
immunocytochemistry
light microscopy

67. Which of the following is not used for detection in GC?
Infrared spectroscopy
NMR
Flame ionisation
Electrical conductivity

68. Which of these effects result from slow injection of a large sample volume?
Increased resolution
Decreased resolution
Non-linear detector response
Constant resolution

69. The GC trace obtained after an experiment is called a
chromatograph
chromatogram
chromatophore
graph

70. Which of the following detectors give concentration-dependent signals?
Electron-capture detector
Thermal conductivity
Infra-red detector
. All of these

71. What useful information can be found from a Van Deemter plot?
The selectivity factor
Optimum mobile phase flow rate
Optimum column temperature
Optimum column length