R4RIN – MCQs, Mock Tests | Biochemistry Biochemistry MCQ Set 2

Arrangement of nucleotides in DNA can be seen using which of the following instruments?

1. Electron microscope
2. Light microscope
3. X-Ray crystallography
4. Ultracentrifuge

✅ Correct Answer: 3

Which of the following involves carrying genetic information from DNA for protein synthesis?

1. sn-RNA
2. r-RNA
3. m-RNA
4. t-RNA

✅ Correct Answer: 3

Which of the following is true about tm?

1. The higher the content of G ≡ C base pairs, the lower the tm
2. The higher the content of A = T base pairs, the higher the tm
3. It can be termed as renaturation temperature
4. The higher the content of G ≡ C base pairs, the higher the tm

✅ Correct Answer: 4

Which of the following is true about the Z-DNA helix?

1. It has fewer base pairs per turn than B-DNA
2. It tends to be found at the 3’ end of the genes
3. It has alternating GC sequences
4. It is a permanent conformation of DNA

✅ Correct Answer: 1

Which of the following temperature best suits the growth and multiplication of transformed cells?

1. 25°C
2. 26°C
3. 37°C
4. 30°C

✅ Correct Answer: 3

Which of the following enzyme is used in PCR?

1. EcoRII
2. EcoRI
3. Taq DNA polymerase
4. HRP

✅ Correct Answer: 3

Which of the following temperature best suits the growth and multiplication of transformed cells?

1. 25°C
2. 26°C
3. 37°C
4. 30°C

✅ Correct Answer: 3

Which of the following enzyme is used in PCR?

1. EcoRII
2. EcoRI
3. Taq DNA polymerase
4. HRP

✅ Correct Answer: 3

Which of the following temperature best suits the growth and multiplication of transformed cells?

1. 25°C
2. 26°C
3. 37°C
4. 30°C

✅ Correct Answer: 3

Recombinant DNA construction involves ____________

1. Cleaving DNA with a restriction endonuclease and joining with polymerase
2. Cleaving and joining DNA with restriction endonuclease
3. Cleaving DNA with a restriction endonuclease and joining with ligase
4. Cleaving DNA with ligase and joining with endonuclease

✅ Correct Answer: 3

Which of the following is termed as conserved gene order?

1. Microarray
2. Ortholog
3. Synteny
4. Paralog

✅ Correct Answer: 3

Meristem culture helps in developing which of the following?

1. Hybrid plants
2. Virus free plants
3. Tall plants
4. Disease resistant plants

✅ Correct Answer: 2

Biochemistry MCQ on Lipids, Biological Membranes and Transport 1. The number of milligrams of KOH required to neutralize the free and combined fatty acid in one gram of a given fat is called __________

1. Polenske number
2. Acid number
3. Saponification number
4. Iodine number

✅ Correct Answer: 3

Which of the following membrane lipids have a direct glycosidic linkage between the head-group sugar and the backbone glycerol?

1. Ether lipids
2. Sphingolipids
3. Glycolipids
4. Phospholipids

✅ Correct Answer: 3

Which of the following hormone is responsible for the activation of phospholipase C?

1. Adrenaline
2. Serotonin
3. Cortisol
4. Vasopressin

✅ Correct Answer: 4

Which of the following membranes contain low cholesterol and high cardiolipin?

1. Cell wall
2. Inner mitochondrial membrane of the hepatocyte
3. Myelin sheath
4. Plasma membrane

✅ Correct Answer: 2

Which of the following is a choline-containing lipid?

1. Phosphatidylethanolamine
2. Phosphatidylserine
3. Sphingomyelin
4. Phosphatidylglycerol

✅ Correct Answer: 3

Which of the following proteins does not function in cell-cell interaction?

1. Cadherin
2. Cytochrome c
3. Integrin
4. N-CAM

✅ Correct Answer: 2

How many types of signal transducers are there?

1. 6
2. 7
3. 8
4. 4

✅ Correct Answer: 1

Which of the following is a intracellular second messengers?

1. Glycine
2. Glutamate
3. Acetylcholine
4. IP3

✅ Correct Answer: 4

Which of the following is not correct about phosphorylation by IRS on serine residue?

1. Target tissues fail to respond to circulating insulin
2. It increases insulin levels
3. It is an inhibitory phosphorylation
4. It is molecular mechanism for insulin resistance

✅ Correct Answer: 1

Which of the following catalyzes the cutting of PIP2 into 2 moles of IP3 and diacylglycerol in cell signaling?

1. Phosphodiesterase
2. Phosphokinase C
3. Phospholipase C
4. Lipokinase

✅ Correct Answer: 3

Which of the following directly phosphorylate tyrosines to start a kinase protein enzymatic cascade?

1. Receptor threonine kinase
2. Receptor serine kinase
3. Receptor tyrosine kinase
4. Receptor guanylyl cyclases

✅ Correct Answer: 3

When visual stimulus reaches receptors in retina of eye, first process involving absorption of physical energy by receptors is?

1. Coding
2. Adaptation
3. Transduction
4. Reception

✅ Correct Answer: 4

Which of the following drug is used to terminate early pregnancies?

1. Victoza
2. Glucophage
3. RU486
4. Metformin

✅ Correct Answer: 3

Which of the following occurs in meiosis but not in mitosis?

1. Pairing of homologous chromosomes at metaphase plate
2. Separation of sister chromatids at anaphase
3. Attachment of spindle fibers to kinetochore
4. Replication of DNA prior to start of cell division

✅ Correct Answer: 1

Migration of cancerous cells from the site of origin to other part of the body forming secondary tumors is called ___________

1. Proliferation
2. Diapedesis
3. Apoptosis
4. Metastasis

✅ Correct Answer: 4

Which of the following is the study of energy relationships and conversions in biological systems?

1. Biochemistry
2. Biotechnology
3. Biotechnology
4. Bioenergetics

✅ Correct Answer: 4

Anabolism and catabolism are chemically linked in the form of __________

1. ASP
2. ADP
3. ATP
4. Phosphodiester linkage

✅ Correct Answer: 3

Which of the following is not a flavoprotein?

1. NADH dehydrogenase-Qreductase
2. Succinate dehydrogenase
3. Xanthine oxidase
4. Cytochrome c

✅ Correct Answer: 4

Whenever the cell’s ATP supply is depleted, which of the following enzyme’s activity is increased?

1. Pyruvate kinase
2. Phosphofructokinase-1
3. Hexokinase
4. Glucokinase

✅ Correct Answer: 2

Whenever the cell’s ATP supply is depleted, which of the following enzyme’s activity is increased?

1. Pyruvate kinase
2. Phosphofructokinase-1
3. Hexokinase
4. Glucokinase

✅ Correct Answer: 2

Which of the following enzyme is responsible for the conversion of glucose 1-phosphate to glucose 6-phosphate?

1. Epimerase
2. Phosphoglucomutase
3. Glycogen phosphorylase
4. Isomerase

✅ Correct Answer: 2

Which is the major factor(s) determining whether glucose is oxidized by aerobic or anaerobic glycolysis?

1. Presence of high AMP
2. NADH and the ATP/ADP ratio
3. Ca+2
4. FADH2

✅ Correct Answer: 2

Which of the following is not an important precursor of glucose in animals?

1. Pyruvate
2. Glucose 6-phosphate
3. Lactate
4. Glycerol

✅ Correct Answer: 2

Which of the following is not an important precursor of glucose in animals?

1. Pyruvate
2. Glucose 6-phosphate
3. Lactate
4. Glycerol

✅ Correct Answer: 2

Which of the following enzymes acts in the pentose phosphate pathway?

1. 6-phosphogluconate dehydrogenase
2. Pyruvate kinase
3. Glycogen phosphorylase
4. Aldolase

✅ Correct Answer: 1

Which of the following is not a factor determining the activity of an enzyme?

1. Nucleotides
2. Association with regulatory protein
3. Sequestration
4. Allosteric regulation

✅ Correct Answer: 1

How many steps are catalyzed by different enzymes in glycolysis and gluconeogenesis?

1. 3
2. 4
3. 1
4. 2

✅ Correct Answer: 1

Which compounds are responsible for the coordinated regulation of glucose and glycogen metabolism?

1. Fructose 2, 6-bisphosphate
2. Acetyl co-A
3. NADH
4. NAD+

✅ Correct Answer: 1

Which of the following is a measure of the effect of an enzyme’s concentration on flux through a multi-enzyme pathway?

1. Metabolic control
2. Response coefficient
3. Elasticity coefficient
4. Flux control coefficient

✅ Correct Answer: 4

Which of the following is a measure of how responsive the enzyme is to changes in the concentration of a metabolite?

1. Metabolic control
2. Response coefficient
3. Flux control coefficient
4. Elasticity coefficient

✅ Correct Answer: 4

Which of the following is a measure of how responsive the enzyme is to changes in the concentration of a metabolite?

1. Metabolic control
2. Response coefficient
3. Flux control coefficient
4. Elasticity coefficient

✅ Correct Answer: 4

Which is the first step in the catabolism of most L-amino acids once they have reached the liver is promoted?

1. Glutamine synthase
2. Glutamate dehydrogenase
3. Glutaminase
4. Amino transferases

✅ Correct Answer: 4

Which of the following operates at an important intersection of carbon and nitrogen metabolism?

1. lutamine synthase
2. Glutaminase
3. Glutaminase
4. Amino transferases

✅ Correct Answer: 2

Which of the following is the first amino group entering into urea cycle?

1. Argininosuccinate
2. Cituilline
3. Carbamoyl phosphate
4. Ornithine

✅ Correct Answer: 3

Which of the following are exclusively glucogenic?

1. Lysine
2. Leucine
3. Threonine
4. Arginine

✅ Correct Answer: 4

Which of the following produces pyruvate?

1. Alanine
2. Leucine
3. Isoleucine
4. Lysine

✅ Correct Answer: 1

Complex 4 is also known as _________

1. Cytochrome oxidase
2. NADH dehydrogenase
3. Succinate dehydrogenase
4. Cytochrome bc1 complex

✅ Correct Answer: 1

Which of the following is the prosthetic group of NADH dehydrogenase?

1. FAD
2. FMN
3. NADH
4. NADPH

✅ Correct Answer: 2

Long-chain fatty acids are oxidized step-wise in one-carbon unit starting from ____________

1. Aliphatic end
2. Carboxyl end
3. a and b
4. None of the mentioned

✅ Correct Answer: 1

Which effects the increased levels of hydrogen ions in the inter-membrane space of the mitochondria?

1. Decreased levels of chemiosmosis
2. Increased levels of water in inter-membrane space
3. Increase ATP production
4. Decreased levels of oxidative phosphorylation

✅ Correct Answer: 3

How many mitochondrial proteins are encoded in nuclear genes and imported into mitochondria after their synthesis?

1. 300
2. 500
3. 700
4. 900

✅ Correct Answer: 4

What is the effect of the excessive rate of apoptosis?

1. AIDS
2. Fever
3. Atrophy
4. Sneezing

✅ Correct Answer: 3

Which one of the following is the source of electrons in photosynthesis? a) NADH b) Water c) Carbohydrates d) Which one of the following is the source of electrons in photosynthesis?

1. NADH
2. Water
3. Carbohydrates
4. CO2

✅ Correct Answer: 2

Which of the following is the correct sequence for the movement of electrons during the light-dependent reactions of plants?

1. P680, water, P700, NADP+
2. Water, P680, P700, NADP+
3. P700, P680, NADP+, water
4. Water, P700, NADP+, P680

✅ Correct Answer: 2

Which of the following condenses acyl and malonyl groups?

1. Acetyl co-A ACP transacetylase
2. Malonyl co-A ACP transferase
3. β-ketoacyl ACP synthase
4. Acyl carrier protein

✅ Correct Answer: 3

Which of the following class of drugs reduce the levels of fatty acids circulating in the blood?

1. Amphetamines
2. Thiazolidinediones
3. Synthetic cannabinoids
4. Cathinones

✅ Correct Answer: 2

Which of the following is involved in the bio-signaling pathway that includes membrane turnover and exocytosis?

1. Phosphatidyl glycerol
2. Phosphatidylinositol
3. Phosphatidyl glycerol and myoinositol
4. Myoinositol

✅ Correct Answer: 2

How many carbons are present in HMG co-A?

1. 12
2. 8
3. 6
4. 4

✅ Correct Answer: 4

Which of the following catalyzes reactions that incorporate nitrogen derived from glutamine?

1. Glutamine synthase
2. Glutamine amidotransferase
3. Adenylyltransferase
4. Glutamate synthase

✅ Correct Answer: 2

Which of the following gives rise to methionine, threonine, and lysine?

1. Glutamate
2. Serine
3. Pyruvate
4. Aspartate

✅ Correct Answer: 4

Which of the following gives rise to γ-amino butyrate?

1. Pyruvate decarboxylation
2. Glutamine synthase
3. Glutamate synthase
4. Glutamate decarboxylation

✅ Correct Answer: 4

Which of the following is degraded to methylmalonyl semialdehyde?

1. Leucine
2. Thymine
3. Glutamine
4. Tyrosine

✅ Correct Answer: 2

Which of the following has the lowest level of chromosome organization?

1. 30nm fiber
2. Nucleosome
3. Solenoid
4. None of these

✅ Correct Answer: 2

Which of the following plays a substantial role in linking together sister chromatids immediately after replication?

1. Topoisomerases
2. Histones
3. Condensins
4. Cohesins

✅ Correct Answer: 4

Which of the following has beads on a string structure?

1. Nucleosomes
2. Chromosomes
3. Heterochromatin
4. Chromatin

✅ Correct Answer: 1

Which of the following histones shows more sequence similarity among eukaryotic species?

1. H2A
2. H3
3. H1
4. H2B

✅ Correct Answer: 2

Which of the following enzymes remove supercoiling in replicating DNA ahead of the replication fork?

1. Topoisomerases
2. Primases
3. Helicases
4. DNA polymerases

✅ Correct Answer: 1

Which of the following mechanisms will remove uracil and incorporate the correct base?

1. Nucleotide excision repair
2. Direct repair
3. Base excision repair
4. Mismatch repair

✅ Correct Answer: 3

Which of the following process occurs in regions where no large–scale sequence similarity is apparent?

1. Site specific recombination
2. Replicative recombination
3. Homologous genetic recombination
4. Non-homologous recombination

✅ Correct Answer: 3

Which of the following is not involved in the post-transcriptional processing of t-RNA?

1. Attachment of poly-A tail
2. Base modulation
3. Attachment of CCA arm
4. Splicing

✅ Correct Answer: 1

Which of the following is an example of RNA-dependent DNA polymerase?

1. RNA polymerase II
2. DNA ligase
3. Reverse transcriptase
4. RNA polymerase I

✅ Correct Answer: 3

Which position of a codon is said to wobble?

1. Fourth
2. First
3. Second
4. Third

✅ Correct Answer: 4

Which of the following inactivates the 60S subunit of eukaryotic ribosomes?

1. Ricin
2. Chloramphenicol
3. Cycloheximide
4. Diphtheria toxin

✅ Correct Answer: 1