The order for the construction of a cDNA fragment from mRNA is to

  1. Home

- Online Exam Test Papers | - MCQs[multiple choice questions and answers ] | - Mock Test Papers | - Practice Papers | - Sample Test Papers |

Question:
The order for the construction of a cDNA fragment from mRNA is to

1. bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase

2.treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT

3.digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase

4.bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase

Posted Date:-2021-03-07 10:53:13

More MCQS Questions and answers

  1. An RNA primer is synthesized by
  2. In order to insert a foreign gene into a plasmid, both must __________
  3. Proteins which help to open the double helix for DNA synthesis are
  4. Which enzyme catalyzes the elongation of DNA molecules?
  5. Which of the following is not true of small inbreeding populations?
  6. During conventional transformation experiments E. coli cells and plasmid DNA interacts in an environment of
  7. Okazaki fragments occur on the __________ and are bonded together by _________
  8. What is the exact name of the classical Watson-Crick double helix DNA?
  9. A human cDNA library
  10. A plasmid
  11. An enzyme that recognizes foreign DNA, and results in a cut in that DNA is called
  12. An example of a restriction fragment length polymorphism is
  13. An expression vector
  14. An important difference between eukaryotic and prokaryotic DNA replication is
  15. Automated DNA sequencing uses the chain termination method
  16. Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because
  17. DNA replication in eukaryotes occurs only in
  18. DNA replication results in
  19. DNA segments capable of moving from one place in the genome to another is known as
  20. DNA with a G-C content of 50% will melt at approximately
  21. First discovered, Type II restriction endonuclease was
  22. First evident that DNA is genetic material comes from the experiment of Griffith is
  23. For the DNA replication in eukaryotes, the cell cycle consists of
  24. he unpaired nucleotides produced by the action of restriction enzymes are referred to have
  25. In an agarose gel, DNA is moving from
  26. In DNA, nucleotides are covalently joined together by
  27. Isoschizomers recognize
  28.  Which of the following species lack a 'classical' histone H1?
  29. Knockout mice are created by
  30. n gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel.
  31. Plant transformation can be obtained by
  32. Replication in E. coli is initiated by the generation of short RNA primers using
  33. Restriction enzymes
  34. Restriction enzymes are named for
  35. Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are
  36. The B-DNA structure found in solution is a
  37. The DNA is negatively super coiled, complexes to histone,
  38. The order for the construction of a cDNA fragment from mRNA is to
  39. The RNA primers used to initiate replication in E. coli
  40. The RP13 gene of chromosome 17 codes for a protein __________ .
  41. The site on a bacterial chromosome that marks the initiation point for chromosome replication is called
  42. The TP53 gene of chromosome 17 codes for a protein __________ .
  43. The transfer of antibiotic-resistant genes from genetically engineered bacteria to disease-causing bacteria __________ .
  44. There is a single origin for DNA replication in bacteria. How many origins of replication are found in the much larger genomes of eukaryotes such as mammals?
  45. TM refers to the temperature at which
  46. Under which of the following conditions would population gene frequencies remain the same?
  47. What does ligase do during replication of DNA?
  48. What enzyme forms covalent bonds between restriction fragments?.
  49. What is the IUPAC code for an A, C, T ambiguity in a nucleic acid sequence?
  50. What is the name given to the points at which a DNA helix is unwound and new strands develop?
  51. When populations are small, gene frequencies can change from generation to generation and some alleles may become fixed in a population. This is called __________ .
  52. Which of the following are short nucleotides strands made by DNA polymerase working in the opposite direction of the replication fork?
  53. Which of the following genetic diseases would be amenable to genetic engineering?
  54. Which of the following in DNA replication and transcription are common?
  55. Which of the following is an active site on its single polypeptide chain for DNA Polymerase I?
  56. Which of the following is correct in terms of determination of location of genetic traits?
  57. Which of the following is correct?
  58. Which of the following is not a structural motif in DNA binding proteins?
  59. Which of the following pair will produce complementary sticky ends?
  60. Which of the following techniques can be used to determine the defective gene and for developing cancer?
  61. Which of these drugs bind to DNA and cuts it, producing hydroxyl radicals?
  62. Which of these genes codes for a protein that plays a role in growth?
  63. Which of these genes codes for a protein that plays a role in white blood cell function?
  64. Which of these restriction enzymes produce blunt ends?
  65. Which polymerase is active in DNA repairing
  66. Which type of restriction enzymes do not usually require ATP?
  67. Which will require a higher temperature to denature?
  68. Who's X-ray work aided Watson and Crick in their discovery of the double helix?
  69. Why is golden rice pale yellow in color?
  70. X-rays cause
Search
R4R Team
R4Rin Top Tutorials are Core Java,Hibernate ,Spring,Sturts.The content on R4R.in website is done by expert team not only with the help of books but along with the strong professional knowledge in all context like coding,designing, marketing,etc!